Hif 1 alpha antibody

This immunoglobulin has been raised against a peptide of interest , binding the antigen with high affinity. Immunogen A portion of amino acids 9-33 from human HIF1A was used as the immunogen for the HIF-1 alpha antibody. The HIF-1 transcription factor drives hypoxic gene expression changes that are thought to be adaptive for cells exposed to a reduced-oxygen environment. 低酸素誘導因子(hif)1αは、低酸素状態に適応する主な遺伝子発現調節因子です。ターゲット遺伝子はvegf、エリスロポエチン、解糖酵素、グルコーストランスポーターおよび血管新生、代謝調節、細胞増殖や生存等に関わる重要な因子が含まれています。按产品名称查看(排序方式:数字-英文字母-希腊字母-中文拼音)Storage Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). HIF-1 alpha was detected with a rabbit monoclonal anti-HIF-1 alpha antibody [EP1215Y] (ab51608) at 1:1,000 dilution. 0. The main action of the VHL protein is thought to be its E3 ubiquitin ligase activity that results in specific target proteins being 'marked' for degradation. . Storage Instruction: Store as concentrated solution. It is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. 2. Each HIF-1 alpha Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. MicroRNA-210 (miR-210) is known to be induced by HIF-1α and can regulate various pathological processes, but its role in hypoxic kidney injury remains unclear. 8. HIF-1 alpha is most closely related to Sim. Each kit provides sufficient reagents to Hypoxia-inducible factor 1-alpha, also known as HIF-1α, is a subunit of the heterodimeric transcription factor hypoxia-inducible factor 1 (HIF-1). Among its related pathways are ERK Signaling and Gene Expression. Collagen‐I antibody (abcam UK, ab34710 1/100 dilution). Cited in 1 publication. 3 antibody Hypoxia inducible factor 1 alpha subunit antibody Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody When oxygen levels are sufficient in tissues, hypoxia-inducible factor-1 (HIF-1)-α and HIF-2α experience posttranscriptional iron- and oxygen-dependent hydroxylation, which occurs on two proline residues within the oxygen-dependent degradation (ODD) domain of the α-subunit. 1. HIV-1 gp41, Rabbit Polyclonal Antibody Product Details Rabbit serum against the E. Formulation: Lyophilized from a 0. Alternative names for HIF2A / HIF2 alpha antibody. The membrane was incubated with rabbit anti-HIF-1-alpha/HIF1A antigen affinity purified polyclonal antibody (Catalog # PA1041) at 0. Western Blot protocol specific for HIF-1 alpha Antibody (NB100-123) Page 1 of 1 HIF-1 Alpha Regulates the Response of Primary Sarcomas to Radiation Therapy through a Cell Autonomous Mechanism Minsi Zhang a, 1 , Qiong Qiu b, 1 , Zhizhong Li b , Mohit Sachdeva b , Hooney Min b , Diana M. of a third alpha-class hypoxia This antibody clone is manufactured by Abcam. Rabbit polyclonal HIF-1 alpha antibody. View specifications, prices, citations, reviews, and Detection of Human, Mouse, and Rat HIF‑1 alpha by Western Blot. 025% ProClin 300 was added as a preservative. Cited in 2 publication(s). Hypoxia-induced gene expression is initiated when HIF-1subunit is stabilized in response to a lack of oxygen. MedKoo is one of the world leading suppliers of anticancer chemical reagents and kinase inhibitors. HIF-1 alpha [EP1215Y] is a rabbit monoclonal antibody that is intended for laboratory use in the qualitative identification of HIF-1 alpha protein by immunohistochemistry (IHC) in formalin-fixed paraffin- BioVision’s HIF-1 alpha ELISA kit is a sandwich ELISA assay for the quantitative measurement of human HIF-1 alpha in serum, plasma and cell culture supernatants. Reconstitution: Reconstitute at 0. It regulates the physiological responses to low oxygen levels (hypoxia) and the pathophysiology of heart attack, cancer, stroke and chronic lung disease. ,biological,biology supply,biology supplies,biology product Hypoxia inducible factor 1 alpha isoform I. The resultant protein is produced in two forms, an 18 kDa and a 30 kDa protein that functions as a tumor suppressor. HIF-1α (Hypoxia-inducible factor 1α,HIF1A) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced O2 availability in mammals, including angiogenesis, erythropoiesis and glycolysis. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism Western Blot of Jurkat cell lysate, nontreated or treated with Cobalt(II) chloride (CoCl2), using Anti-HIF-1-alpha RM242 at a 1:1000 dilution, showed that HIF-1-alpha (~120 kDa) expression was induced by CoCl2 in Jurkat cells. 2 μm filtered solution in PBS with BSA as a carrier protein. The RAS-transformed cell lines MDFB6 and B6ras show near complete loss of TSP-1 expression. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. HIF-1beta (hypoxia-inducible factor-1 beta) Antibody Review . Find MSDS or SDS, a COA, data sheets and more information. HIF-1 alpha Antibody (H-206) is a high quality rabbit polyclonal recommended for detecting HIF-1 alpha of mouse, rat and human origin by WB, IP, IF and ELISA Choose a Store Santa cruz biotechnology Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). 1-1 mg/mL in sterile PBS containing at least 0. HIF-1 functions as master regulator of response to oxygen homeostasis. Diseases associated with VEGFA include Microvascular Complications Of Diabetes 1 and Poems Syndrome. It was shown previously that HIF-1beta protein expression is nearly ubiquitous in newborn kidney and that HIF-1beta dimerizes with either HIF-1alpha or -2alpha. 1,2 Following hypoxic stimulus and cytoplasmic accumulation, HIF-1α migrates to the nucleus where with other transcription factors, it drives the production of stress-adaptive proteins. HIF‑1 alpha was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Mouse Anti-Human/Mouse HIF‑1 alpha Monoclonal Antibody (Catalog # MAB1935) at 15 µg/mL overnight at 4 °C. HIF1 alpha antibody (hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)) for ICC/IF, IHC-Fr, IHC-P, IP, WB, ChIP assay. Angiogenesis is a complex and highly coordinated process that ultimately leads to the formation of …Welcome to Medkoo Biosciences. 00元: 加入购物车: d3118: 生物素随机引物dna标记试剂盒VEGFA (Vascular Endothelial Growth Factor A) is a Protein Coding gene. Anti-HIF-1-alpha unpurified antibody (ab51608) reactivity with reduced Hep3B cell lysate after transient transfection of scrambled siRNA (lanes1-3 and 7-9) or HIF-1-alpha siRNA (lanes 4-6 and 10-12). HIF-1 alpha is most closely related to Sim (2). 1272/2008 1. Ramos cells (Burkitt’s lymphoma cell line) were treated with CoCl 2. HIF‐1 alpha antibody (abcam UK, ab2185 1/200 dilution). General principles of angiogenesis. Hypoxia-inducible factor 1 alpha, HIF-1 alpha, ARNT-interacting protein, Member of PAS protein 1, Basic-helix-loop-helix-PAS protein MOP1, HIF1A, MOP1 Target Function: Functions as a master transcriptional regulator of the adaptive response to hypoxia. HIF-1 alpha antibody has been shown to upregulate several genes to promote survival in hypoxic environments. Rabbit polyclonal HIF-1 alpha antibody. HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. The most researched of these targets is hypoxia inducible factor 1a (HIF1a), a transcription factor that induces the Our 10,000-square-foot R&D facility is located at Wuxi City, China (one hour drive west to Shanghai) and is equipped with state-of-the-art chemistry labs and advanced analytical instruments including Agilent HPLC/LCMS and Bruker 400MHz NMR. Me mahdollistamme tieteen tekemisen tarjoamalla käyttöösi kattavan tuotevalikoiman, hyvän palvelun, toimivat prosessit ja osaavat ihmisemme. Q16665, C0LZJ3, Q53XP6, Q96PT9, Q9UPB1. The antibody was produced by immunization of rabbits with purified recombinant influenza A/New Caledonia/20/99 produced in insect cells using baculovirus expression vector system. Product name Anti-HIF-1-alpha antibody [ESEE122] Description Mouse monoclonal [ESEE122] to HIF-1-alpha Specificity This antibody is specific for HIF-1-alpha. Pathological angiogenesis2. 1…View Mouse TNF-alpha Quantikine ELISA Kit (MTA00B) datasheet. Lisätietoa aiheesta Anti-HIF-1-alpha Rabbit Polyclonal Antibody. Resolve aliquots (25-30 µg) of induced nuclear protein extracts on a 4-20 % Tris-HCl gel. GENERAL INFORMATION Immunogen: Recombinant Human HIF-1 alpha / HIF1A Protein (Catalog#11977-H07E) Preparation This antibody was obtained from a rabbit immunized with purified, recombinant Human HIF-1 alpha / This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. Request a Quote from Novus Biologicals. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. HIF-1 alpha is a rabbit monoclonal antibody derived from cell culture superna- tant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7. . Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300- interacting transactivator. HIF-1 mediates these effects by inhibiting C-MYC activity via two mechanisms. The HIF-1 transcription factor drives hypoxic gene expression changes that are thought to be adaptive for cells exposed to a reduced-oxygen environment. For immunofluorescence, sections were pretreated with previously, then incubated in the mixture of anti- HIF-1α antibody/anti-GFAP(mouse, 1:200, Proteintech), anti-HIF-1α antibody (rabbit, Abcam, 1:50)/anti-NeuN (mouse, 1:200, Abcam), After washed with PBS, they were incubated with fluorescence secondary antibody IgG (anti-mouse Alexa Fluor The mutant HIF-1 probe showed no spe- assay was performed using these nuclear lysates and cific complex formation (Fig. Doctors prescribe Desmopressin most frequently for treatment of diabetes insipidus or nocturnal enuresis. IHC analysis of HIF 1 alpha using anti-HIF 1 alpha antibody (PB9253). (B) HL-1 cells transfected with hypoxia-inducible factor-1 alpha (HIF-1α), haem oxygenase-1 (HMOX-1), or empty vector (pcDNA3. Compare Anti-HIF1 alpha Antibody Products from leading suppliers on Biocompare. HIF-1a transcription activity assay HIF-1a transcriptional activity was analyzed by HIF-1a transcription factor assay using TransAM HIF-1 transcription factor assay kit (Active Motif, Carlsbad, CA) according to the manufacturers instructions. 2). labelled oligonucleotides containing either the consensus We studied the transcriptional activity of HIF-1 by binding site for HIF-1 or its mutated version as a control. HIF-1alpha (hypoxia-inducible factor-1 alpha) Antibody Review . Titration of the HIF-1 alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity. This antibody reacts with Fish, Hamster, Human, Mouse, Non-human primate, Ovine, Rat samples. hif 1 alpha antibodyBiological Strategies: Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Cited in 14 publication(s). Hypoxia-Inducible Factor 1 alpha (HIF-1-alpha) Antibody Staining Protocol for Immunohistochemistry . Western blot shows lysates of MCF‑7 human breast cancer cell line, Balb‑3T3 mouse HIF-1 alpha Antibody (H1 alpha 67) is a mouse monoclonal recommended for detecting HIF-1 alpha of mouse, rat and human origin by WB, IP, IF and IHC(P)Rabbit recombinant monoclonal HIF-1 alpha antibody [EP1215Y]. Gene. Introduction . 产品编号: 产品名称: 产品包装: 产品价格: 操作: d3106: 生物素3' 末端dna标记试剂盒: 20次: 1368. Expression of Iron Regulatory Protein 1 Is Regulated not only by HIF-1 but also pCREB under Hypoxia Massachusetts, USA; and primary anti-HIF-1 alpha antibody from Purification and Characterization of Hypoxia-Inducible Factor. Tested applications Flow Cyt, ICC/IF, ELISA, IHC-Fr, IHC-P Species reactivity Reacts with: Mouse, Rat, Cow, Human Immunogen Recombinant fragment corresponding to Human HIF-1-alpha aa 329-530. dependent asparagine hydroxylation of Hypoxia-inducible Factor Alpha R-HSA-1234176 Oxygen-dependent proline Anti-human HIF-lα antibody (BD Pharmingen, USA) (1:800) was used as a primary antibody and mouse-Ig conjugated horseradish peroxidase (1:3,000) was used as a secondary antibody. 25 M sodium chloride and 0. HIF1-alpha Antibody Gene and Protein Information Hypoxia-inducible factor-1 (HIF1) is a basic helix-loop-helix (bHLH)-PER-ARNT-sim (PAS) transcription factor that plays a role in the cellular response to hypoxia. The primary antibody was used Anti-HIF-1-alpha [H1alpha67] antibody (ab1) at 1/200 dilution in PBS and incubated with This Image is courtesy of an anonymous Abreview sample at 4°C for 12 hours. hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) Background: The alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. The remaining supernatant was incubated overnight at 4 °C with 2 μg of the HIF-1<alpha> primary antibody or 1-μl negative control IgG antibody. Background of HIF1A / HIF1 alpha antibody. Immunogen. 1 mM for 4 hours), using HIF-1α Antibody. H. 1; NP_690008. Storage: Antibody can be stored for 6 months -20° C to -70° C. All HIF-1 alpha reagents are produced in house and quality controlled, including 4 HIF-1 alpha Antibody, 15 HIF-1 alpha Gene, 1 HIF-1 alpha Protein, 1 HIF-1 alpha qPCR. One of the mechanisms underlying this phenomenon is cell apoptosis triggered by hypoxia-inducible factor-1- α (HIF-1α) activation. Powell J, Elshtein R, Forest D, Palladino M. Cells were incubated at with 21% O 2 (lanes 1-6) or 1% O 2 (lanes 7-12) for 4h before lysis. Mouse tissue was used as a control. The kidney is vulnerable to hypoxia-induced injury. Co-IP assays using anti–HIF-1α antibody for hypoxia-inducible factor 1 that is required for breast cancer progression. 5-1. Original magnification: ×100. 5 μg/mL overnight at 4°C, then washed with TBS-0. Effect of Silymarin on Lipid and Alcohol Metabolism The HIF-1 transcription factor drives hypoxic gene expression changes that are thought to be adaptive for cells exposed to a reduced-oxygen environment. Immunogen corresponding to synthetic peptide. Hypoxia inducible factor 1 alpha isoform I. Immunohistochemistry: HIF-1 alpha, mAb (H1alpha67) [Prod. 1) (Uniprot Accession #A6VGJ4). Both subunits are constantly translated. This antibody reacts with human, mouse, rat, bovine, canine. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes Hypoxia inducible factor-1(HIF-1) is a transcription factor composed of two subunits namely HIF-1α and HIF-1β. This antibody also works for ChIP. A synthetic peptide corresponding to a sequence in the middle region of human HIF-2-alpha(282-301aa, HALDSENMTKSHQNLCTKGQ), identical to the related mouse and rat sequence. Quality Assurance Evaluated by Western Blot HIF-1 alpha Antibody (28b) is a high quality mouse monoclonal recommended for detecting HIF-1 alpha of human origin by WB, IP, IF and IHC(P) KLN 205 cells labeled with Rabbit HIF-1 Alpha Polyclonal Antibody Polyclonal Antibody, Unconjugated (bs-0737R) followed by a FITC Conjugated Secondary Antibody. Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. Learn more about Anti-HIF-1 alpha Mouse Monoclonal Antibody (Alexa Fluor® 700) [clone: ESEE122]. Hypoxia-Inducible Factor 1-Alpha, HIF-1-Alpha, HIF1-Alpha, ARNT-Interacting Protein, Basic-Helix-Loop-Helix-PAS Protein MOP1, Class E Basic Helix-Loop-Helix Protein 78, bHLHe78, Member of PAS Protein 1, PAS Domain-Containing Protein 8, HIF1A, BHLHE78, MOP1 Note: HIF-1 alpha Antibody SKU: NB100-654 for targeting HIF-1 alpha – Novus Biologicals Novus Biologicals 's HIF-1 alpha Antibody SKU: NB100-654 is a primary antibody of unspecified isotype. Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Hypoxia-inducible factor 1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A_HUMAN. Mouse anti-HIF-1α antibody reacts specifically with HIF1 α subunit of sheep, mouse, rat, ferret and human. Cardona c , Thomas F. 5 hour at RT. Purified Mouse Anti-Human HIF-1α Clone 54/HIF-1α HIF-1 α. Predicted to react with Rat, Mouse, Zebra Fish, and Bovine based on 100% sequence homology. Monoclonal, polyclonal, and recombinant antibodies. This gene was mapped to 14q21-q24. Journal of Biological Chemistry. 5 hours at room temperature. Desmopressin acetate is a synthetic replacement for vasopressin, the hormone that reduces urine production. Anti-HIF1 alpha Antibody Products HIF1 alpha is an alias for the gene, Hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), in humans. HIF1 is a heterodimer composed of an alpha s Alpha in fixed cells. The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody [GT10211] (GTX628480) at a dilution of 1:500. First, HIF-1 binds to and activates transcription of the MXI1 gene, which encodes a repressor of C-MYC tran- scriptional activity. Our HIF-1 alpha Antibodies can be used in a variety of model species: Avian, Bovine, Canine, Chicken, Ferret, Goat, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Primate, Rabbit, Rat, Sheep, Xenopus, Yeast. 5, containing BSA and sodium azide as a preservative. This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. The ELISA Genie HIF-1 alpha / HIF1A ELISA Kit can assay for HIF-1 alpha / HIF1A in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary at 1/500 dilution. The HIF-1 alpha Antibody Pack from Novus Biologicals is a set of mouse antibodies to HIF-1 alpha. Cedarlane provides high quality research reagents for the life science community mouse anti-HIF2 alpha Antibody, mouse anti-HIF2a Antibody, ECYT4, EPAS1, HIF2alpha, HIF1 Alpha like factor, HLF, Hypoxia inducible factor 2 alpha, MOP2, PASD2, Endothelial PAS domain-containing protein 1, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73 Hypoxia-Inducible Factor 1-Alpha, HIF-1-Alpha, HIF1-Alpha, ARNT-Interacting Protein, Basic-Helix-Loop-Helix-PAS Protein MOP1, Class E Basic Helix-Loop-Helix Protein 78, bHLHe78, Member of PAS Protein 1, PAS Domain-Containing Protein 8, HIF1A, BHLHE78, MOP1 Note: HIF-1-Alpha overexpression is associated with increased proliferation, which might explain the adverse prognostic impact of increased concentrations of HIF-1-alpha in invasive breast cancer. Validated in WB, IP, IHC, Flow Cyt, ICC/IF and tested in Human. Liu, C. BACKGROUND Hypoxia-inducible factor-1 (HIF-1) is a dimeric transcriptional complex that has been recognized primarily for its role in the maintenance of oxygen and energy homoeostasis. 1% sodium azide. HIF-1 alpha, also known as HIF1A, contains 1 basic helix-loop-helix (bHLH) domain, 1 PAC (PAS-associated C-terminal) domain and 2 PAS (PER-ARNT-SIM) domains. Transfer proteins to nitrocellulose membrane in 20 mM Tris-HCL (pH 8. Ki67 antibody (abcam UK, ab15580 1/200 dilution). HIF-1 alpha was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Mouse Anti-Human/Mouse HIF-1 alpha Monoclonal Antibody (Catalog # MAB1935) at 15 ╡g/mL overnight at 4 C. Anti-β tubulin antibody (BD, Pharmingen, USA) was used as a control. Need antibody products for research? Find and compare multiple sources of anti-HIF-1 Alpha antibody using the Linscott's Directory search engine. C. In addition, administration of the antibody prolonged survival of SCID mice implanted with JOK-1 cells. (B) HIF-1alpha protein expression in 775 products 5 reviews. Get complete supplier details here. Bio-Synthesis provide quality Cell Penetrating Peptide, Antibody Polyclonal Production, Peptide Molecule Production and Melanocyte Stimulating Hormones Hypoxia-inducible factor 1-alpha. Select applications, conjugates, hosts, and reactivity. All HIF-1 alpha reagents are ready to use. Detection of Human, Mouse, and Rat HIF‑1 alpha by Western Blot. Further Information. The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). AbMiner is a development of the Genomics and Pharmacology Facility, Developmental Therapeutics Branch (DTB), Center for Cancer Research (CCR), National Cancer Institute (NCI). (A) Adipose hypoxia examination with pimonidazole staining in HFD mice. Western blot analysis of HIF3 alpha expression in CoCl2 treated 3T3 cell lysates (lane 1) and 3T3 cell lysates (lane 2). Description: HIF-1α Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Please Note: Optimal concentrations/dilutions should be determined by the end user. 3 antibody Hypoxia inducible factor 1 alpha subunit antibody Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody Important Comments This anti-HIF3 alpha antibody is expected to recognize isoform a and c (NP_690007. Mouse anti Human HIF1 alpha antibody, clone Halpha111a recognizes human Hypoxia-inducible factor 1-alpha otherwise known as HIF1 alpha (HIF1A) or ARNT interacting protein, a widely expressed bHLH-PAS transcription factor, which acts as a critical regulatory protein in the host response to hypoxia (Kubis et al. Protein Names. 3 antibody Hypoxia inducible factor 1 alpha subunit antibody Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody HIF‐1 activation is iron‐independent and occurs through the PKC and PI‐3K pathways. Anticorps, Protéines recombinantes, coffret ELISA, RNAi, clones ADNc, Antibody Array, coffret Luminex. GPER is a transcriptional target of hypoxia inducible factor 1 alpha (HIF-1 α) and activates VEGF expression and angiogenesis in hypoxic breast tumor microenvironment. The HIF-1 alpha Antibody (ESEE122) [FITC] from Novus Biologicals is a mouse monoclonal antibody to HIF-1 alpha. 3 antibody Hypoxia inducible factor 1 alpha subunit antibody Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody Use Anti-HIF-1-alpha Antibody, hydroxyproline (Pro402) (Rabbit Polyclonal Antibody) validated in WB to detect HIF-1-alpha also known as Hypoxia-inducible factor 1-alpha, HIF1-alpha. The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. These antibodies react with human, mouse, rat, primate. HIF1 alpha antibody [GT10211] detects HIF1 alpha protein by western blot analysis. The density of color is proportional to the amount of Learn more about Anti-HIF-1 alpha Mouse Monoclonal Antibody (DyLight 755) [clone: H1alpha67]. 1907/2006 as amended by (EC) No. Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information ( NCBI ) website for more information about accession numbers and Hydroxylates HIF-1 alpha at Asn-803 in the C-terminal transactivation domain (CAD). Example blot. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. Hypoxia -Inducible Factor-1αHypoxia-inducible factor-1α (HIF-1α) is a transcription factor that accumulates under low-oxygen conditions. Buy anti-HIF-1 alpha Rabbit Polyclonal Mouse antibody 11977-RP02. Non-hypoxic pathway mediates the induction of hypoxia inducible factor 1 alpha (HIF-1α) in vascular smooth muscle cells. (C-Term) Polyclonal Antibody SAFETY DATA SHEET Supersedes Revision: 08/02/2011 according to Regulation (EC) No. Chen, M. Hypoxia-Inducible Factor 1-Alpha (HIF-1-alpha or HIF1A) is an important mediator of the cellular and systemic responses to hypoxia, promoting survival and maintaining homeostasis in conditions of low oxygen. Antigen Info Immunogen Type HIF3 alpha antibody peptide antigen is from the N terminus region of HIF3A and had the following sequence: QDHRSTTELRKEKS Collagen‐I antibody (abcam UK, ab34710 1/100 dilution). Image kindly submitted by Edita Aksamitiene as part of the Free Sample Program. BHLHE73, HIF-2 alpha, MOP2, PASD2, Hypoxia-inducible factor 2 alpha, HIF-1 alpha-like factor, HLF, HIF-related factor, EPAS1, Endothelial PAS domain-containing protein 1, Class E basic helix-loop-helix protein 73, Basic-helix-loop-helix-PAS protein MOP2 Compare & find the top performing anti-Human Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) antibody for Substrate (S). Mouse anti Human HIF1 alpha antibody, clone ESEE122 recognizes human HIF1α, also known as Hypoxia-inducible factor 1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1 or PAS domain-containing protein 8. Rat HIF-1 Alpha (Hypoxia Inducible Factor 1 Alpha) ELISA Kit. Human HIF-1 alpha (isoform 1) has a predicted length of 826 residues and a MW of 93 kDa. 0 ug/mL is recommended to detect HIF1A in formalin fixed and paraffin embedded tissues. Nichols a , Christopher 6. HIF 1A, HIF-1a, HIF1 alpha, HIF1A. Validated for ELISA. Caspase‐3 antibody (abcam UK, ab13847 1/100 dilution). Human HIF-1 alpha / HIF1A ELISA Kit - Information. Y. The hypoxia inducible factor (HIF-1) is a transcription factor composed of an inducible alpha form and the constitutively expressed beta form, also called the aryl hydrocarbon receptor nuclear translocator (ARNT). Species Reactivity Notes Demonstrated to react with Human. Introduction. anti-Hif-1alpha (human), mAb antibody (ANC10G3) (ANC-335-820) is a monoclonal antibody specific for human Hif-1alpha, working in flow cytometry and ELISA and important for hypoxia and cancer research. 0)/150 mM glycine/20 % (vol/vol) methanol. 2005). no. 1. for hypoxic stabilization of HIF-1 alpha nor Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS Antibody Validation for HIF-1-alpha in Equine Hoof Tissue - Life Sciences bibliographies - in Harvard style Figure 1. Hypoxia-inducible factor 1-alpha (HIF1 alpha) is a constitutively expressed transcription factor that is degraded under normal oxygen tensions but stabilized when oxygen is limiting (hypoxia). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). Jimin Shin, Hyo-Jeong Lee, Deok-Beom Jung, Ji Hoon Jung, Hyo-Jung Lee, Eun-Ok Lee, Seok Geun Lee, Beom Sang Shim, Seung Hoon Choi, Seong Gyu Ko HIF-2α-IN-1 is a HIF-2α inhibitor has an IC50 of less than 500 nM in HIF-2α scintillation proximity assay. HIF-1 alpha is a nuclear protein that activates gene transcription in response to reduced cellular O2 concentration. Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric basic-helix-loop-helix-PAS transcription factor consisting of HIF-1 alpha and HIF-1 beta subunits. HIF-1 alpha Antibody (28b) is a high quality mouse monoclonal recommended for detecting HIF-1 alpha of human origin by WB, IP, IF and IHC(P) This antibody recognizes HIF-1-alpha at the ODD region. 1 Product Code: 10006421 Section 1. HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. Second, HIF-1 promotes MXI-1-independent, proteasome-dependent degrada- tion of C-MYC. HIF1 Alpha antibody LS-B1503 is an unconjugated rabbit polyclonal antibody to HIF1 Alpha (HIF1A) from human, mouse, rat and other species. HIF‑1 alpha was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFO but is not detected in HIF‑1 alpha knockout (KO) HeLa Human Cell Line cell line using Mouse Anti-Human/Mouse/Rat HIF‑1 alpha Monoclonal Antibody (Catalog # MAB1536) at 8 µg/mL for 3 hours at room temperature. We enable science by offering product choice, services, process excellence and our people make it happen. alpha. The density of color is proportional to the amount of human HIF-1 alpha captured from the samples. BML-SA287] - Staining of HIF1 alpha in human kidney. HIF-1 alpha can be a difficult target to work with so we have compiled a summary of all the important information you need to know including useful tips. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Properties Form The chromatin immunoprecipitation (ChIP) assay has become one of the most useful the hypoxia-inducible factor 1 alpha Anti-HIF-1 Antibody May be stored for up This antibody can be used at 1:1000-1:2000 with the appropriate secondary reagents to detect Human HIF1A. This occurs as a posttranslational modification by prolyl hydroxylation. Product information "Anti-HIF1A (HIF-1 alpha)" Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. 1) were subjected to 300 µM H 2 O 2 for 4 h. HIF1A. for HIF-1 alpha antibody (pAb) Background Hypoxia-inducible factor-1 (HIF1) is a transcription factor involved in the regulation of oxygen homeostasis. Validated in WB, ELISA, IHC and tested in Mouse, Human. UniProt Code History. REAGENTS AND METHODS FOR DIAGNOSING CONDITIONS ASSOCIATED WITH HYDROXYLATED HIF 1-ALPHA An antibody of claim 1 comprising a variable region of a light Mouse Anti-HIF-1 beta Monoclonal Antibody, Unconjugated, Clone 29 from BD Biosciences Pharmingen,HIF-1 beta (ARNT1) Purified Anti-Mouse, Anti-Rat, Anti-Human clone 29, Isotype Mouse IgG , 50 µg Consult technical datasheet for details. The protease inhibitor cocktail II supernatant (1%) from each sample was saved before the IP and served as the total input chromatin. Anxiety Disorders, Cardiovascular Diseases, Congenital Abnormalities, Drug Toxicity, Fibrosis, Heart Defects, Congenital, Kidney Diseases, LEFT VENTRICULAR NONCOMPACTION 1, Neoplasms, Nervous System Diseases are some of the diseases may be linked to Recombinant Human Dystrobrevin alpha (DTNA). HIF1A, BHLHE78, MOP1, PASD8 Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Staining of HIF1 alpha in human kidney. This immunoglobulin has been raised against HIF-1 alpha , binding the antigen with high affinity. Hsu, H. Institute of Signaling, Developmental Biology and Cancer Research UMR CNRS 6543, Centre Antoine Lacassagne, 33 Avenue Valombrose, 06189 Nice, FRANCE. Darren E. E. 02 M phosphate buffer containing 0. In mice ischemic limbs, a gene medicine (pSC-HIF1αtri/L64) composed of L64 and pSC-based expression plasmid encoding hypoxia-inducible factor 1-alpha triple mutant (HIF-1αtri), improved the expression of stable HIF-1α, and in turn, the expression of multiple angiogenic factors. 7. Immunogen KLH-conjugated linear peptide corresponding to the ODD region of human HIF-1-alpha. 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. , Y. A concentration of 0. Physical form Solution in 0. Cells were lyzed in Laemmli Buffer and 10 µg was loaded into a 10% SDS-PAGE. HIF2-Alpha is involved in catecholamine homeostasis, vascular remodeling, physiological angiogenesis and adipogenesis. Hypoxia-inducible factor 1-alpha (HIF1A), ELISA Kit The ELISA analytical biochemical technique of the MBS2885065 kit is based on HIF1A antibody-HIF1A antigen ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF1, HIF1 ALPHA, HIF1A, HIF-1α, Member of PAS protein 1, MOP1, PASD8 Background HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear Anti-HIF-1α (K377) Antibody Description: Rabbit polyclonal antibody to HIF-1α (K377) The expressions of alpha smooth muscle actin (α-SMA), collagen I, CD31 and HIF-1 alpha Protein NBC1-18422 has been successfully used for antibody preadsorption/blocking assay in IHC-P with Hif-1alpha antibody NB100-479 (refer to product review by Yves Heremans on the datasheet of NBC1-18422). Chang, and C. Thioredoxin-1 (Trx-1) is a small redox protein overexpressed in a number of human primary HIF-1 alpha, also known as HIF1A, contains 1 basic helix-loop-helix (bHLH) domain, 1 PAC (PAS-associated C-terminal) domain and 2 PAS (PER-ARNT-SIM) domains. HIF-beta subunits are stable regardless of oxygen concentration and constitutively translocate to the nucleus. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 µg) were separated by 7. Identification of the Substance/Mixture and of the Company/Undertaking Product Name: HIF-1. is essential for The monoclonal antibody was purified from tissue culture supernatant or ascites Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). Validated in WB, IHC, Flow Cyt, ICC/IF and tested in Mouse, Rat, Human. HIF-1 alpha Antibody (H1alpha 67-7) for targeting a peptide of interest – Novus Biologicals Novus Biologicals 's HIF-1 alpha Antibody (H1alpha 67-7) is a primary antibody of unspecified isotype. Polyclonal Antibody for studying HIF1A in the Protein Translation research area. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. 3 antibody Hypoxia inducible factor 1 alpha subunit antibody Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody Hypoxia inducible factor 1 alpha isoform I. HIF-1 Alpha (EP118) Rabbit Monoclonal Antibody Updated: 2017-05-24 08:46:09 The HIF-1 Alpha subunit of hypoxia-inducible factor 1 is a transcription factor that functions as a master transcriptional regulator of the adaptive response to hypoxia. Finally the coverslips were mounted with ProLong ® Gold Antifade with DAPI (Life Technologies, USA). coli-derived recombinant human HIF-1α Arg575-Asn826 Accession # Q16665. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Oxygen-breathing species express this highly-conserved transcriptional complex. Members of the hypoxia-inducible factor (HIF) family of transcription factors regulate the cellular response to hypoxia (1). Wash the membrane in water for 5 min and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1. (C-Term) Polyclonal Antibody HIF-1 alpha (556-574) Description: This is a hypoxia-inducible factor-1 19-mer fragment. Independently reviewed in 1 Polyclonal Antibody for studying HIF1A in the Protein Translation research area. The hypoxia-inducible factors (HIF) are alpha/beta heterodimeric transcription factors of the basic helix-loop-helix-Per-Arnt-Sim (bHLH-PAS) superfamily and are chiefly responsible for cellular adaptation to oxygen deprivation. Elisa Conde, Laura Alegre, Ignacio Blanco-Sánchez, David Sáenz-Morales, Elia Aguado-Fraile, Belén Ponte, Edurne Ramos, Ana Sáiz, Carlos Jiménez, Angel Ordoñez 1:1000 dilution of the Mouse Anti- HIF-1α antibody. HIF1AN: Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Desmopressin (1-desamino-8-D-arginine vasopressin) is a modified form of the normal human hormone arginine vasopressin, a 近期學術著作: 期刊論文. The pheT phet product has the following accession number(s) (GI #500695369) (NCBI Accession #WP_011976902. * Your Name hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) Background This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. Independently reviewed in 1 Biological Strategies: Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Ilkayeva g , Ki Lui a , Amanda G. Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). Un-treated (-) and treated (+, 500 μM CoCl2 treatment for 24hr) HeLa whole cell extracts (30 µg) were separated by 7. Fill in the form to request a quote from Novus Biologicals about (NB100-123) HIF-1 alpha Antibody (H1alpha67) [Mouse Monoclonal]. Tested applications Flow Cyt, ELISA, IHC-Fr, IHC-P, WB Species reactivity Reacts with: Rat, Human Immunogen Fusion protein (proprietary-tag) corresponding to Human HIF-2-alpha aa 535-631. Richard*, Edurne Berra, and Jacques Pouysségur. Western blot shows lysates of MCF‑7 human breast cancer cell line, Balb‑3T3 mouse Anti-HIF-1-alpha unpurified antibody (ab51608) reactivity with reduced Hep3B cell lysate after transient transfection of scrambled siRNA (lanes1-3 and 7-9) or HIF-1-alpha siRNA (lanes 4-6 and 10-12). Image from verified 775 products 5 reviews. Iron is required for HIF‐1 degradation in normoxic cells, as it is a necessary cofactor for prolyl and asparginyl hydroxylases , and so decreased iron availability activates HIF‐1 . Avoid repeated freeze-thaw cycles. The HIF-1 alpha Antibody (ESEE122) [FITC] has been validated for the following applications: Immunocytochemistry / Immunofluorescence, Immunohistochemistry-Paraffin Description The Thermo Scientific HIF-1 alpha ELISA Kit contains sufficient reagents and buffers to assay HIF-1 alpha (hypoxia-inducible factor 1-alpha) levels in serum, plasma (EDTA, heparin, sodium citrate), and culture lysate. HIF-1 consists of a constitutively expressed subunit HIF-1β and an oxygen-regulated subunit HIF-1α (or its paralogs HIF-2α and HIF-3α). HIF-1 binds to cis-acting hypoxia-response elements within the erythropoietin, vascular endothelial growth factor, and other genes to activate transcription in hypoxic cells. hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) Background This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-134] - Analysis of HIF-1 alpha on Zebrafish retina tissue using HIF-1 alpha antibody. Product NB100-105 is recommended for most Western blot experiments. It may be taken nasally, intravenously, or as a tablet. Description Mouse monoclonal [ep190b] to HIF-2-alpha Specificity This antibody is specific for HIF-2-alpha and does not cross-react with HIF-1-alpha. 270(3): 1230-1237, 1995. Tested on 20 paraffin-embedded human tissues. The antibody is a rabbit polyclonal antibody raised against HIF2a. HIF-1 is found in mammalian cells cultured under reduced O2 tension and is necessary for transcriptional activation mediated by the erythropoietin gene enhancer in Providing an extensive range of internationally recognized polyclonal antibodies designed for research use. Apoptosis induction appears to be regulated by the hypoxia inducible factor 1α pathway. Cetuximab inhibits HIF-1-controlled glycolysis in cancers cells, thereby reversing the Warburg Background Human immunodeficiency disease type 1 (HIV-1) induces neuronal dysfunction through Archives HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. hif 1 alpha antibody 4A, lanes 9 and 10). Hypoxia-inducible factor 1 (HIF-1), a heterodimer of HIF-1 alpha and HIF-1 beta subunits, is a transcriptional activator central to the cellular response to low oxygen that includes metabolic adaptation, angiogenesis, metastasis, and inhibited apoptosis. Cited in 83 publication(s). Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining. The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains. **This antibody has demonstrated varying results in Western blot applications. Hypoxia Inducible Factor 1-Alpha (HIF-1 Alpha) Is Induced during Reperfusion after Renal Ischemia and Is Critical for Proximal Tubule Cell Survival. Figure 1 Effects of Rg5 on adipose hypoxia. Suppression of STAT3 and HIF-1 Alpha Mediates Anti-Angiogenic Activity of Betulinic Acid in Hypoxic PC-3 Prostate Cancer Cells. DeLaney f , Tracy Han b , Yan Ma b , Lixia Luo b , Olga R. We previously reported that cetuximab, a Meals and Medication Administration-approved anti-epidermal development aspect receptor (EGFR) antibody, exerts its antitumor activity at least partly via inhibiting the Warburg impact through downregulating hypoxia-inducible aspect-1 alpha (HIF-1) [6-8], the regulatory subunit of HIF-1, which really is The antibody is a rabbit polyclonal antibody raised against HIF2a. Data for anti hif 1 alpha antibody ep1215y gathered from related PubMed articles. Chiu. BioVision’s HIF-1 alpha ELISA kit is a sandwich ELISA assay for the quantitative measurement of human HIF-1 alpha in serum, plasma and cell culture supernatants. Transglutaminase 2 is involved in the inflammatory response through mechanisms linked to NF-kappa B/HIF-1 alpha pathways TG2 protein was co-immunoprecipitated with PLA2 by monoclonal antibody The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Bulk in stock. Image from verified Rabbit recombinant monoclonal HIF-1 alpha antibody [EP1215Y]. The antigen was purified under conditions that preserve the HA proteins biological activity and tertiary structure. Based on 100% sequence identity, this antibody is expected to react with: Cavia porcellus, Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Grass carp, Northern pike, Atlantic cod, Duckbill platypus and Gansu zokor. Immunogen The immunogen recognized by this antibody maps to a region between residues 775 and the C-terminus (residue 826) of human hypoxia-inducible factor 1 (Q16665) Clonality Polyclonal NCBI Full Gene Name hypoxia inducible factor 1 subunit alpha HIF-1 Alpha (EP118) Rabbit Monoclonal Antibody Updated: 2017-05-24 08:46:09 The HIF-1 Alpha subunit of hypoxia-inducible factor 1 is a transcription factor that functions as a master transcriptional regulator of the adaptive response to hypoxia. Image from verified customer review. Validated for IHC and WB. Our 10,000-square-foot R&D facility is located at Wuxi City, China (one hour drive west to Shanghai) and is equipped with state-of-the-art chemistry labs and advanced analytical instruments including Agilent HPLC/LCMS and Bruker 400MHz NMR. Hypoxia-inducible factor 1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A Need antibody products for research? Find and compare multiple sources of anti-HIF-1 Alpha antibody using the Linscott's Directory search engine. HIF-1β is a constitutively nuclear-located subunit that is found under all oxygen conditions. Has been shown to detect HIF-1 alpha in both mouse and human tissue by IHC with Citrate buffer antigen retrieval. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HIF-1 alpha / HIF1A (rh HIF-1 alpha / HIF1A; Catalog#11977-H07E; Q16665-1; Arg575-Asn826). HIF 1 alpha was detected in paraffin-embedded section of human intestinal cancer tissue. Chou C. Dilute the mouse anti-HIF-1 alpha primary antibody (NB 100-449) 1:500 in blocking buffer and incubate overnight at 4C. HIF1-ALPHA antibody hypoxia inducible factor 1 alpha subunit antibody hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) antibody BACKGROUND Hypoxia-inducible factor-1 (HIF-1) is a dimeric transcriptional complex that has been recognized primarily for its role in the maintenance of oxygen and energy homoeostasis. Clioquinol, the Cu/Zn-chelator, inhibits ubiquitination through preventing hydroxylation at Asn-813. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and protein heterodimerization activity. coli derived recombinant HIV-I protein, DEV-I (contains the C- terminus of gp120 and most of gp41). The HIF1A gene encodes the HIF-1alpha subunit of hypoxia-inducible factor 1, a transcription factor that is essential for cardiovascular development and systemic O2 homeostasis (1). The antibody can be stored at 2° - 8° C for 1 month. Western blot analysis of extracts from Raji and Jurkat cells, untreated or treated with cobalt chloride (0. "The Human Mouse Rat HIF 1 alpha Antibody from R D Systems is a mouse monoclonal antibody to HIF 1 alpha This antibody reacts with human mouse rat The Human Mouse Rat HIF 1 alpha Antibody has been validated for the following applications Western Blot Simple Western Immunoprecipitation Knockout Validated" Synonyms: HIF-1alpha antibody, HIF1 antibody, HIF1-ALPHA antibody, MOP1 antibody, PASD8 antibody, bHLHe78 antibody, HIF1A antibody, basic-helix-loop-helix-PAS protein MOP1 antibody, class E basic helix-loop-helix protein 78 antibody, ARNT-interacting protein antibody, member of PAS superfamily 1 antibody, hypoxia-inducible factor 1, alpha The primary factor mediating this response is the hypoxia-inducible factor-1 (HIF-1), an oxygen-sensitive transcriptional activator. We previously reported that cetuximab, a Meals and Medication Administration-approved anti-epidermal development aspect receptor (EGFR) antibody, exerts its antitumor activity at least partly via inhibiting the Warburg impact through downregulating hypoxia-inducible aspect-1 alpha (HIF-1) [6-8], the regulatory subunit of HIF-1, which really is Western Blot protocol specific for HIF-1 alpha Antibody Protocol specific for NB100-123 Monoclonal Anti-HIF-1 alphaWestern Blot Protocol 1. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. It has been selected for its ability to recognize HIF2a in immunohistochemical staining and western blotting. It is one of the two subunits of Hypoxia-inducible factor-1 (HIF1). Furthermore, IGF1/IGF1R signaling, which has angiogenic effects, has been shown to activate GPER in breast cancer cells. Gene Synonyms. Page: 1 of 6 HIF-1. HIF-2 alpha Polyclonal Antibody from Invitrogen for Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Flow Cytometry, Immunoprecipitation, ChIP assay, ELISA and Gel Shift applications. •Alpha-Ketoglutarate •Fe 2+ •Ascorbic acid •Oxygen Transcriptional co-activators: •p300 •CBP - Creb binding protein •SRC-1 - Steroid receptor co-activator 1 •TIF-2 - Transcription intermediary factor 2 •Ref-1 - Redox factor 1 Produced in collaboration with Claudie Hooper, King’s College London HIF αpathway Detection with HIF-1α or HMOX-1 antibody and Alexa488-labelled secondary antibody. Ultrasound-guided RNA interference targeting HIF-1 alpha improves the effects of transarterial chemoembolization in rat liver tumors Cheng-Shi Chen,1,2,* Qing Zhao,1,* Sheng Qian,1 Hai-Liang Li,2 Chen-Yang Guo,2 Wei Zhang,1 Zhi-Ping Yan,1 Rong Liu,1 Jian-Hua Wang1 1Department of Interventional Radiology, Zhongshan Hospital, Fudan University Anti-angiogenic effects of SN38 (active metabolite of irinotecan): inhibition of hypoxia-inducible factor 1 alpha (HIF-1α)/vascular endothelial growth factor (VEGF) expression of glioma and growth of endothelial cells This antibody was used to detect HIF-1alpha expression in control (NTC) and HIF-1alpha knockdown subclone in response to carboplatin treatment in human breast cancer cell line by western blot. HIF-1 Alpha ELISA Kit, 480 Assays, Human Species, Immunology Research Category, Colorimetric Detection Method, Colorimetric Elisa (TMB Substrate) in Clear 96-Well Strip Plate, The Complete Kits Contain Precoated 96-Well Strip Plate(S) and Lot-Optimized Re Basic helix loop helix PAS protein MOP2; Basic-helix-loop-helix-PAS protein MOP2; bHLHe73; Class E basic helix-loop-helix protein 73; ECYT4; Endothelial PAS domain containing protein 1; Endothelial pas domain protein 1; Endothelial PAS domain-containing protein 1; EPAS 1; EPAS-1; EPAS1; EPAS1_HUMAN; HIF 1 alpha like factor; HIF 2 alpha; HIF-1 Abcam anti hif 1 alpha antibody ep1215y Bioz Stars score: 98/100, Techniques, Protocol Conditions and more for anti hif 1 alpha antibody ep1215y, supplied by Abcam. There is evidence that tumor hypoxia promotes metastasis through the induction of MET overexpression by HIF-1 alpha. HIF1 consists of a constitutively expressed HIF-1β subunit (87 kDa) and an O2-regulated HIF-1α subunit (120 kDa) which contains an O2-dependent degradation domain (ODD). Stimulation of hypoxia-inducible factor-1 alpha (HIF-1alpha) protein in the adult rat testis following ischemic injury occurs without an increase in HIF-1alpha messenger RNA expression. View specifications, prices, citations, reviews, and HIF1A Polyclonal Antibody from Invitrogen for Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry HIF-1 alpha Monoclonal Antibody from Invitrogen for Immunofluorescence, Immunocytochemistry and Immunohistochemistry (Paraffin) applications. HIF3 alpha at 72 kDa was detected using rabbit anti-HIF3 alpha affinity purified polyclonal antibody (Catalog # A03805) at 1:1,600. Click here to view our high-quality products